Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1985-6-25
pubmed:abstractText
We propose a role for GTP hydrolysis in microtubule assembly in which the GTPase reaction serves to stabilize tubulin subunits in the microtubule. The GTPase reaction in tubulin subunits containing GTP at microtubule ends is presumed to occur predominately in subunits at one of the interfaces between a cap of GTP-containing tubulin subunit and a core of GDP-containing tubulin subunit in the microtubule, resulting in elongation of the core. The proposed model interprets the effects of GDP on microtubule assembly, using a reaction scheme in which GDP-containing tubulin subunits are able to add to microtubule ends. The model can account for the GTP requirement for microtubule assembly, the GDP inhibition of the rate for microtubule elongation, and the fact that a metastable state exists after the enzymic conversion of GTP to GDP, with microtubules which are at steady state. To account for the fact that the microtubule assembly and disassembly rates are nonlinearly dependent upon the tubulin subunit concentration and for the effects of GDP-containing tubulin subunits on the kinetic properties of microtubules, our scheme includes nonproductive as well as productive binding of GTP- and GDP-containing tubulin subunits. We compare our model with an alternative scheme [Hill, T. L. & Carlier, M. F. (1983) Proc. Natl. Acad. Sci. USA 80, 7234-7238], which interprets the effects of GDP on microtubule assembly using a reaction scheme in which GDP is able to exchange with GTP in GTP-containing tubulin subunits in the microtubule and in which the principal GTPase occurs in GTP-containing tubulin subunits at the microtubule/solution interface.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-1003481, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-511939, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-5419909, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6102089, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-620419, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-656371, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6580643, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6583675, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6584870, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6592585, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6643473, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6755216, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6822568, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6849867, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6933529, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6940174, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-6943550, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7000766, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7026576, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7061471, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7074050, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7074077, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7103973, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7225365, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7240159, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-728162, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7298643, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7326342, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7350166, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7408905, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7411636, http://linkedlifedata.com/resource/pubmed/commentcorrection/3858823-7459331
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3267-71
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Directed elongation model for microtubule GTP hydrolysis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.