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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-6-6
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pubmed:abstractText |
Clinical observations have shown that hypercholesterolemia is associated with abnormal androgen metabolism, viz. an increased excretion of etiocholanolone (E) relative to androsterone (A). Substances which restore the A/E ratio to normal likewise lower serum cholesterol. Postulating that the abnormal steroid and sterol metabolism may be either causally related or dependent on the same metabolic defect, we have developed in vitro and in vivo models to select drugs which favorably effect the ratio of A to E produced from [4-14C]androst-4-ene-3,17-dione [4-14C]A-dione). The in vitro model employs a mixture of rat liver microsomal delta 4-3-ketosteroid-5 alpha-reductase and cytosolic 3 alpha-hydroxysteroid dehydrogenase and delta 4-3-ketosteroid-5 beta-reductase. Kinetic and mechanistic studies have been performed on active compounds using this in vitro assay. The in vivo model employs i.v. injection of [4-14C]A-dione followed by collection of bile in anesthetized, hypophysectomized female rats. Many compounds preselected in the in vitro assay likewise reduced the A/E ratio in vivo. One of these compounds (CGS 10614A) also lowered serum cholesterol and reduced the incidence and severity of atherosclerotic lesions in aortas of cholesterol-fed rabbits.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9150
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-36
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:3857915-3-Hydroxysteroid Dehydrogenases,
pubmed-meshheading:3857915-3-Oxo-5-alpha-Steroid 4-Dehydrogenase,
pubmed-meshheading:3857915-3-alpha-Hydroxysteroid Dehydrogenase (B-Specific),
pubmed-meshheading:3857915-Androstenedione,
pubmed-meshheading:3857915-Androsterone,
pubmed-meshheading:3857915-Animals,
pubmed-meshheading:3857915-Arteriosclerosis,
pubmed-meshheading:3857915-Bile,
pubmed-meshheading:3857915-Cytosol,
pubmed-meshheading:3857915-Etiocholanolone,
pubmed-meshheading:3857915-Female,
pubmed-meshheading:3857915-Hypolipidemic Agents,
pubmed-meshheading:3857915-Hypophysectomy,
pubmed-meshheading:3857915-Liver,
pubmed-meshheading:3857915-Microsomes, Liver,
pubmed-meshheading:3857915-Oxidoreductases,
pubmed-meshheading:3857915-Rats
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pubmed:year |
1985
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pubmed:articleTitle |
Identification of potential antiatherosclerotic/hypolipidemic agents by their effect on hepatic conversion of androst-4-ene-3,17-dione to etiocholanolone and androsterone.
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pubmed:publicationType |
Journal Article
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