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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1979-12-29
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pubmed:abstractText |
Peritoneal macrophages from the endotoxin-unresponsive C3H/HeJ substrain of mice were entirely refractory to activation in vitro by protein-free LPS, a defect that was not overcome by co-culture of spleen cells from the responder C3H/St substrain with LPS resistant C3H/HeJ macrophages. The defect in responsiveness appears confined to the lipid A activation signal since C3H/HeJ macrophages were fully activated after in vitro treatment by lipid A protein (LAP)--LPS complex, isolated LAP, and BCG. Moreover, after exposure to allogeneic tumor cells in vivo, C3H/HeJ macrophages were cytotoxic for tumor target cells in vitro. By contrast, macrophages from the responder C3H/St strain were fully activated by protein-free LPS to become cytolytic for tumor cells in vitro. C3H/HeJ macrophages, therefore, exhibit a highly selective defect characterized by unresponsiveness to the lipid A activation signal of protein-free LPS and resistance to the toxic effects of high concentrations of LPS that were lethal to the responder C3H/St strain.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
123
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2304-10
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:385780-Animals,
pubmed-meshheading:385780-Cell Differentiation,
pubmed-meshheading:385780-Cells, Cultured,
pubmed-meshheading:385780-Female,
pubmed-meshheading:385780-Immunologic Memory,
pubmed-meshheading:385780-Lipid A,
pubmed-meshheading:385780-Lipopolysaccharides,
pubmed-meshheading:385780-Macrophages,
pubmed-meshheading:385780-Mice,
pubmed-meshheading:385780-Mice, Inbred C3H,
pubmed-meshheading:385780-Mycobacterium bovis,
pubmed-meshheading:385780-Phenols,
pubmed-meshheading:385780-Selection, Genetic,
pubmed-meshheading:385780-Spleen
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pubmed:year |
1979
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pubmed:articleTitle |
Macrophage stimulation by bacterial lipopolysaccharides. III. Selective unresponsiveness of C3H/HeJ macrophages to the lipid A differentiation signal.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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