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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1985-6-20
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pubmed:abstractText |
We hybridized Raji Burkitt lymphoma cells, which carry a t(8;14) chromosome translocation, with human lymphoblastoid cells to study the expression of the translocated cellular myc oncogene (c-myc) in the hybrid cells. In Raji cells the c-myc oncogene is translocated to a switch region of the gamma heavy chain locus (S gamma). Because of sequence alterations in the 5' exon of the translocated c-myc oncogene in this cell line, it is possible to distinguish the transcripts of the translocated c-myc gene and of the normal c-myc gene. S1 nuclease protection experiments with a c-myc first exon probe indicate that Raji cells express predominantly the translocated c-myc gene, while the level of expression of the normal c-myc gene is less than 2% of that of the translocated c-myc gene. Somatic cell hybrids between Raji and human lymphoblastoid cells retain the lymphoblastoid phenotype and express only the normal c-myc oncogene. This result indicates that the activation of a c-myc oncogene translocated to a S region depends on the stage of B-cell differentiation of the cells harboring the translocated c-myc gene and not on alterations in the structure of the translocated c-myc oncogene.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-114999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-1174504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-206891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-3838203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-3856319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-3921823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6308654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6324175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6327285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6328505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6334305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6356357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6401867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6402776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6414084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6416334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6417658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6419123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6424112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6424234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6547209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6818551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6956887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3857623-6961453
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2900-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3857623-Base Sequence,
pubmed-meshheading:3857623-Burkitt Lymphoma,
pubmed-meshheading:3857623-Cell Differentiation,
pubmed-meshheading:3857623-Cell Line,
pubmed-meshheading:3857623-Chromosomes, Human, 13-15,
pubmed-meshheading:3857623-Chromosomes, Human, 6-12 and X,
pubmed-meshheading:3857623-DNA, Neoplasm,
pubmed-meshheading:3857623-Humans,
pubmed-meshheading:3857623-Hybrid Cells,
pubmed-meshheading:3857623-Lymphocytes,
pubmed-meshheading:3857623-Oncogenes,
pubmed-meshheading:3857623-Transcription, Genetic,
pubmed-meshheading:3857623-Translocation, Genetic
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pubmed:year |
1985
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pubmed:articleTitle |
The translocated c-myc oncogene of Raji Burkitt lymphoma cells is not expressed in human lymphoblastoid cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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