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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1985-5-3
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pubmed:databankReference |
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pubmed:abstractText |
We have constructed a library of Clostridium pasteurianum DNA cloned in the plasmid pBR322. Based on the known amino acid sequence for C. pasteurianum ferredoxin, a 64-fold degenerate heptadecanucleotide pool was synthesized. This mixed probe hybridized to two clones which were shown to contain greater than 6 kilobase pairs of the same genomic DNA. Sequence analysis of a common Sau3A1 0.6-kilobase-pair fragment revealed that it contains the information for the apoferredoxin structural gene. According to the DNA sequence, the only post-translational processing of this small apoprotein is the hydrolysis of the initiator methionine. Putative transcription and translation start and stop signals are present within the sequence.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-118435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-14071565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-14236643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-14293020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-4208644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-421272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-4550392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-4565527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-4573349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-4670202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6087283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6181373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6193737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6269055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6269067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6274853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6304620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6316278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6345200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6369317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6411717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6725206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6793235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-6793593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-7048258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-7228873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-7397188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3856844-94251
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1653-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3856844-Base Sequence,
pubmed-meshheading:3856844-Cloning, Molecular,
pubmed-meshheading:3856844-Clostridium,
pubmed-meshheading:3856844-Codon,
pubmed-meshheading:3856844-DNA, Bacterial,
pubmed-meshheading:3856844-Ferredoxins,
pubmed-meshheading:3856844-Genes, Bacterial,
pubmed-meshheading:3856844-Operon,
pubmed-meshheading:3856844-Protein Biosynthesis,
pubmed-meshheading:3856844-Transcription, Genetic
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pubmed:year |
1985
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pubmed:articleTitle |
Cloning and nucleotide sequence determination of the Clostridium pasteurianum ferredoxin gene.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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