Linked Life Data

3856690

Source:http://linkedlifedata.com/resource/pubmed/id/3856690

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-5-23
pubmed:abstractText
The bacteriophage P1 recombinase Cre mediates site-specific recombination between loxP sites. The loxP site consists of two 13 base-pair inverted repeats separated by an eight base-pair spacer region. When DNA containing the loxP site is incubated with Cre, specific cleavages occur within the spacer region, creating a six base-pair staggered cut. The cuts are centered on the axis of dyad symmetry of the loxP site, resulting in a 5' protruding terminus: 5' A decreases T-G-T-A-T-G C 3' T A-C-A-T-A-C increases G. At the point of cleavage, Cre becomes covalently attached to a 3' PO4, and produces a free 5' OH. A series of experiments were carried out in which a radioactively labeled loxP site is recombined with an unlabeled loxP site to locate the point at which strand exchange takes place during recombination. The points of strand exchange coincide with the sites at which Cre cleavage of the DNA backbone had been detected.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-2836
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
181
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
351-62
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Mechanism of strand cleavage and exchange in the Cre-lox site-specific recombination system.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.