Linked Life Data

3855621

Source:http://linkedlifedata.com/resource/pubmed/id/3855621

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1985-3-14
pubmed:abstractText
The effects of RHC 80267, (1-6-di(O-carbamoyl)cyclohexanone oxime)hexane, a diacylglycerol (DG) lipase inhibitor, on the DG lipase pathway and on arachidonic acid (AA) metabolism were investigated in the iris muscle. Incubation of the iris for 30 min at 37 degrees resulted in a loss of AA from phosphatidylinositol, phosphatidylcholine, and phosphatidylethanolamine of 40, 25, and 32% respectively. It was found that the drug inhibited the activity of DG lipase in the iris microsomal fraction and it increased the accumulation of DG, AA and other glycerolipids in iris muscle prelabeled with [14C]AA, presumably by inhibiting this enzyme. Under the same experimental conditions, the drug increased the accumulation of DG and AA in the tissue in a dose- and time-dependent manner, and it inhibited the synthesis of prostaglandin E2 (PGE2) and PGF2 alpha by iris and iris microsomes in a dose-dependent manner. The data presented indicate that RHC 80267 has nonspecific effects on glycerolipid and AA metabolism in this tissue. We conclude that, while the drug does inhibit DG lipase in the intact iris, the present findings that it increased the accumulation of glycerolipids and AA and that it inhibited the biosynthesis of PGs in this tissue throw some doubt on its use in studies on the mechanism of AA release from membrane phosphoinositides for PG synthesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
539-44
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Effects of (1,6-di(O-carbamoyl)cyclohexanone oxime)hexane (RHC 80267) on prostaglandin biosynthesis and accumulation of diacylglycerol and arachidonic acid in rabbit iris.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.