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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1986-1-6
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pubmed:abstractText |
Rigor complexes between actin and myosin have been shown to cause increased binding of Ca2+ to troponin C. A similar effect of force-generating crossbridges has been suggested as an explanation for the coupling between load and activation which has been observed in skeletal and cardiac muscle. The goal of this study was to test the hypothesis that Ca2+-troponin affinity during crossbridge cycling is load-dependent. Ca2+-binding to detergent-extracted rabbit psoas fibres was measured during ATP-induced force generation and in the relaxed state. To compare Ca2+ binding in the latter two states it was necessary to establish conditions in which ATP-induced force could be regulated independently of free Ca2+ concentration. Such conditions were obtained by the use of either the ATPase inhibitor sodium vanadate or the substitution of MgITP for MgATP as an energy source. This study showed that in the presence of MgATP (or MgITP) the amount of Ca2+ bound to the myofilaments at a given free Ca2+ concentration was independent of the force generated. Thus force per se is not a determinant of Ca2+-troponin affinity.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0142-4319
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
477-86
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3851810-Animals,
pubmed-meshheading:3851810-Calcium,
pubmed-meshheading:3851810-Detergents,
pubmed-meshheading:3851810-Glycerol,
pubmed-meshheading:3851810-Kinetics,
pubmed-meshheading:3851810-Muscle Contraction,
pubmed-meshheading:3851810-Muscle Relaxation,
pubmed-meshheading:3851810-Muscles,
pubmed-meshheading:3851810-Octoxynol,
pubmed-meshheading:3851810-Polyethylene Glycols,
pubmed-meshheading:3851810-Rabbits,
pubmed-meshheading:3851810-Surface-Active Agents,
pubmed-meshheading:3851810-Troponin,
pubmed-meshheading:3851810-Troponin C,
pubmed-meshheading:3851810-Vanadates,
pubmed-meshheading:3851810-Vanadium
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pubmed:year |
1985
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pubmed:articleTitle |
The binding of calcium to detergent-extracted rabbit psoas muscle fibres during relaxation and force generation.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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