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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
26
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pubmed:dateCreated |
1985-12-13
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pubmed:abstractText |
This study addresses whether transcriptional control of the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene in rat liver plays a role in determining the level of reductase mRNA. Isolated rat liver nuclei were allowed to elongate nascent RNA transcripts in the presence of [alpha-32P]CTP, and radiolabeled nuclear reductase RNA was quantitated by filter hybridization. Rats fed a diet supplemented with the drugs cholestyramine and mevinolin and having 20-60-fold induced levels of reductase mRNA exhibited levels of reductase transcription which were 20-fold higher than in rats fed an unsupplemented diet. Over 90% of the transcription of the reductase gene was inhibited by concentrations of alpha-amanitin which selectively inhibit RNA polymerase II. Administration of mevalonolactone (the end product of the reaction catalyzed by reductase) to rats fed cholestyramine and mevinolin caused an 80% decrease in the rate of reductase transcription by approximately 1 h. We conclude that under these conditions changes in reductase transcription are primarily responsible for the regulation of reductase mRNA levels.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amanitins,
http://linkedlifedata.com/resource/pubmed/chemical/Cholestyramine Resin,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxymethylglutaryl CoA Reductases,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxymethylglutaryl-CoA...,
http://linkedlifedata.com/resource/pubmed/chemical/Lovastatin,
http://linkedlifedata.com/resource/pubmed/chemical/Mevalonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Naphthalenes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Polymerase II,
http://linkedlifedata.com/resource/pubmed/chemical/mevalonolactone
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
260
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14363-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:3850901-Amanitins,
pubmed-meshheading:3850901-Animals,
pubmed-meshheading:3850901-Cell Nucleus,
pubmed-meshheading:3850901-Cholestyramine Resin,
pubmed-meshheading:3850901-Hydroxymethylglutaryl CoA Reductases,
pubmed-meshheading:3850901-Hydroxymethylglutaryl-CoA Reductase Inhibitors,
pubmed-meshheading:3850901-Liver,
pubmed-meshheading:3850901-Lovastatin,
pubmed-meshheading:3850901-Male,
pubmed-meshheading:3850901-Mevalonic Acid,
pubmed-meshheading:3850901-Microsomes, Liver,
pubmed-meshheading:3850901-Naphthalenes,
pubmed-meshheading:3850901-Nucleic Acid Hybridization,
pubmed-meshheading:3850901-RNA, Messenger,
pubmed-meshheading:3850901-RNA Polymerase II,
pubmed-meshheading:3850901-Rats,
pubmed-meshheading:3850901-Rats, Inbred Strains,
pubmed-meshheading:3850901-Transcription, Genetic
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pubmed:year |
1985
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pubmed:articleTitle |
Transcriptional regulation of the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene in rat liver.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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