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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1985-12-16
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pubmed:abstractText |
Cytosolic creatine kinase isoenzymes MM, MB, and BB are assembled from M or B subunits which occur in different relative amounts in specific tissues. The accumulation of mRNAs encoding the M and B subunits was measured during myogenesis in culture. The relative concentration of the two mRNAs was determined by hybridization with a M-CK cDNA probe isolated previously and a B-CK cDNA probe, the cloning and characterization of which is reported here. The B-CK cDNA hybridizes specifically to a 1.6-kb mRNA found in brain and gizzard but not in adult skeletal muscle tissue. The M-CK cDNA hybridizes to a smaller mRNA 1.4-kb long which is specific to skeletal muscle. In culture, the B-CK mRNA is transiently induced and then declines to a low but detectable level.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0012-1606
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
112
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
84-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3840441-Animals,
pubmed-meshheading:3840441-Base Sequence,
pubmed-meshheading:3840441-Chick Embryo,
pubmed-meshheading:3840441-Cloning, Molecular,
pubmed-meshheading:3840441-Creatine Kinase,
pubmed-meshheading:3840441-DNA,
pubmed-meshheading:3840441-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3840441-Heart,
pubmed-meshheading:3840441-Isoenzymes,
pubmed-meshheading:3840441-Nucleic Acid Hybridization,
pubmed-meshheading:3840441-Protein Biosynthesis,
pubmed-meshheading:3840441-RNA, Messenger
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pubmed:year |
1985
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pubmed:articleTitle |
Accumulation of creatine kinase mRNA during myogenesis: molecular cloning of a B-creatine kinase cDNA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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