Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1985-5-20
|
pubmed:abstractText |
Bovine adrenal medullary cells have been cultured on microbeads which are placed in a low-volume flow system for measurements of stimulation-response parameters. Electronically controlled stream switching allows stimulation of cells with pulse lengths from 1 s to many minutes; pulses may be repeated indefinitely. Catecholamines secreted are detected by an electrochemical detector downstream from the cells. This flow-injection analysis technique provides a new level of sensitivity and precision for measurement of kinetic parameters of secretion. A manual injection valve allows stimulation by higher levels of stimulant in the presence of constant low levels of stimulant. Such experiments show interesting differences between the effects of K+ and acetylcholine on cells partially desensitized to acetylcholine.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0003-2697
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
144
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
218-27
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:3838628-Adrenal Medulla,
pubmed-meshheading:3838628-Animals,
pubmed-meshheading:3838628-Benzaldehydes,
pubmed-meshheading:3838628-Catecholamines,
pubmed-meshheading:3838628-Cattle,
pubmed-meshheading:3838628-Cells, Cultured,
pubmed-meshheading:3838628-Electrochemistry,
pubmed-meshheading:3838628-Fluorescent Dyes,
pubmed-meshheading:3838628-Glass,
pubmed-meshheading:3838628-Kinetics,
pubmed-meshheading:3838628-Microspheres
|
pubmed:year |
1985
|
pubmed:articleTitle |
Flow-injection analysis of catecholamine secretion from bovine adrenal medulla cells on microbeads.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|