Linked Life Data

3837612

Source:http://linkedlifedata.com/resource/pubmed/id/3837612

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-9-16
pubmed:abstractText
Allergens from castor beans (CB-1A) were fractionated by ion-exchange chromatography both on CM- and SP-Sephadex, employing pH gradient. SP-Sephadex showed better results and fractionated the allergens into 7 components, eluted in a narrow pH range (5.0 - 6.6). An attempt to isolate the major acidic fraction of the group involved successive chromatographic steps on the same gel. RC-13, the isolated fraction, proved homogeneous by rechromatography on SP-Sephadex and isolectric focusing with pH range 3.0 - 10.0 polyacrylamide gel. The pl of RC-13 determined by the latter is approximately 4.2 and allergenic activity was demonstrated by heterologous passive cutaneous anaphylaxis in mice using anti-CB-1A rabbit serum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0001-3765
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
409-15
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Fractionation of castor bean allergens (CB-1A). Isolation of RC-13, a homogeneous allergenic fraction.
pubmed:publicationType
Journal Article