Linked Life Data

3836703

Source:http://linkedlifedata.com/resource/pubmed/id/3836703

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-9-18
pubmed:abstractText
We present a modification of a previously described radioenzymatic technique (Snyder et al., Journal of Pharmacology and Experimental Therapeutics, 153: 544-549, 1966) for the determination of histamine. This assay is based on the incubation of tissue histamine with a partially purified preparation of histamine-N-methyltransferase (S-adenosyl-L-methionine: histamine N-methyltransferase, E.C. 2.1.1.8; HMT) in the presence of the natural donor of methyl groups, [3H]-methyl-S-adenosyl-L-methionine ([3H]-SAMe). We have found that HMT retains its histamine N-methylating activity at 4 degrees C. Incubation at low temperature results in a considerable increase in the sample to blank ratio. The reduction of the total amount of [3H]-SAMe used and addition of anhydrous sodium sulfate before the last organic extraction step further reduced the blank levels. With these modifications, the sensitivity of the method was increased to the lower femtomole range. The present assay can be used for the determination of as little as 1 to 2 pg of histamine in samples from individual rat brain nuclei containing 2 to 10 micrograms of protein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0100-879X
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
409-14
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Femtomole isotopic-enzymatic microassay of histamine in microgram amounts of brain tissue.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't