Linked Life Data

3831363

Source:http://linkedlifedata.com/resource/pubmed/id/3831363

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-5-12
pubmed:abstractText
Human type-A erythrocytes were lysed and solubilized with 0.3% sodium deoxy cholate (DOC). The solubilized membranes were coated onto human type-O glutaraldehyde fixed erythrocytes. The coated erythrocytes were found to agglutinate specifically by anti-A antibodies. Furthermore, solubilized membranes could be successfully coated to non-fixed erythrocytes after dialysing out the DOC. Quantitative absorption assays showed that the density of type-A blood group antigen on the coated erythrocytes was higher than the density of this antigen on human type-A erythrocytes. Erythrocytes that are coated with cell membrane antigens can be used to assess specific antibody titres and also can be applied for a sensitive selection and separation of rosetting lymphocytes that secrete specific antibodies against these antigens.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0141-2760
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
195-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Solubilization of cell-membrane-antigen and its coupling to fixed and non-fixed erythrocytes.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't