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pubmed:abstractText |
Two cysteines of the major neuronal beta-tubulin subunit (beta 1) can be specifically cross-linked with the bifunctional sulfhydryl reagent N',N'-ethylenebis(iodoacetamide) after removal of GTP. A cysteine in position 12 cross-links with one of the cysteines in position 201 or 211. Although the two cross-linked cysteines are separated by at least 189 residues in the primary structure of tubulin, they are maximally 9 A apart in the tertiary structure.
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