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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1987-4-22
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pubmed:abstractText |
Bacterially synthesized, recombinant-DNA-derived bovine growth hormone (r-bGH), prepared as described in the preceding paper in this journal, has been characterized in comparison with pituitary bovine growth hormone (pit-bGH). The characterization criteria include sodium dodecyl sulfate/polyacrylamide gel electrophoresis, automated N-terminal sequence analysis, amino acid composition, isoelectric focusing, reverse-phase high-performance liquid chromatography, ultraviolet absorbance, analysis for free protein thiol, sizing by gel filtration, circular dichroism, radioimmunoassay and biological activity in the hypophysectomized rat weight-gain assay. In every respect the r-bGH appears to be virtually identical to pit-bGH.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
323-30
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:3816808-Amino Acids,
pubmed-meshheading:3816808-Animals,
pubmed-meshheading:3816808-Chromatography, High Pressure Liquid,
pubmed-meshheading:3816808-Circular Dichroism,
pubmed-meshheading:3816808-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3816808-Female,
pubmed-meshheading:3816808-Growth Hormone,
pubmed-meshheading:3816808-Isoelectric Focusing,
pubmed-meshheading:3816808-Radioimmunoassay,
pubmed-meshheading:3816808-Rats,
pubmed-meshheading:3816808-Rats, Inbred Strains,
pubmed-meshheading:3816808-Recombinant Proteins,
pubmed-meshheading:3816808-Spectrophotometry, Ultraviolet,
pubmed-meshheading:3816808-Sulfhydryl Compounds
|
pubmed:year |
1987
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pubmed:articleTitle |
Recombinant-DNA-derived bovine growth hormone from Escherichia coli. 2. Biochemical, biophysical, immunological and biological comparison with the pituitary hormone.
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pubmed:publicationType |
Journal Article,
Comparative Study
|