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pubmed-article:3815852pubmed:dateCreated1987-4-10lld:pubmed
pubmed-article:3815852pubmed:abstractTextA new method for sensitive measurement of delta-aminolaevulinic acid (ALA) in biological material is described. ALA is derivatized with dansyl chloride, separated by HPLC and estimated using a fluorescence detector. The pretreatment of biological samples includes desamination of L-alpha-aminoacids with L-aminoacid-oxidase before dansylation. The sensitivity of the method is slightly below 1 pmol/injection for standards and the lower limit of quantification is 0.1 mumol/l for plasma and 10 nmol/l for cerebrospinal fluid. Reference values in plasma are 3.53 +/- 1.75 (SD) (n = 43) mumol/l and in packed erythrocytes they ranged from 6 to 26 mumol/l (mean: 14.0 +/- 5.5 mumol/l). In cerebrospinal fluid of non-porphyric individuals less than 2 nmol/l were recovered.lld:pubmed
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pubmed-article:3815852pubmed:pagination11-8lld:pubmed
pubmed-article:3815852pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:3815852pubmed:year1986lld:pubmed
pubmed-article:3815852pubmed:articleTitleMeasurement of 5-aminolaevulinic acid by reversed phase HPLC and fluorescence detection.lld:pubmed
pubmed-article:3815852pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:3815852pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed