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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1987-4-10
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pubmed:abstractText |
A new method for sensitive measurement of delta-aminolaevulinic acid (ALA) in biological material is described. ALA is derivatized with dansyl chloride, separated by HPLC and estimated using a fluorescence detector. The pretreatment of biological samples includes desamination of L-alpha-aminoacids with L-aminoacid-oxidase before dansylation. The sensitivity of the method is slightly below 1 pmol/injection for standards and the lower limit of quantification is 0.1 mumol/l for plasma and 10 nmol/l for cerebrospinal fluid. Reference values in plasma are 3.53 +/- 1.75 (SD) (n = 43) mumol/l and in packed erythrocytes they ranged from 6 to 26 mumol/l (mean: 14.0 +/- 5.5 mumol/l). In cerebrospinal fluid of non-porphyric individuals less than 2 nmol/l were recovered.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0009-8981
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3815852-Aminolevulinic Acid,
pubmed-meshheading:3815852-Chromatography, High Pressure Liquid,
pubmed-meshheading:3815852-Dansyl Compounds,
pubmed-meshheading:3815852-Erythrocytes,
pubmed-meshheading:3815852-Humans,
pubmed-meshheading:3815852-Lead Poisoning,
pubmed-meshheading:3815852-Levulinic Acids,
pubmed-meshheading:3815852-Reference Values,
pubmed-meshheading:3815852-Spectrometry, Fluorescence
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pubmed:year |
1986
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pubmed:articleTitle |
Measurement of 5-aminolaevulinic acid by reversed phase HPLC and fluorescence detection.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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