Linked Life Data

3815725

Source:http://linkedlifedata.com/resource/pubmed/id/3815725

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-4-13
pubmed:abstractText
The pharmacokinetics of methotrexate (MTX) in the proliferating and the maturing myeloid compartments of the bone marrow and in the neutrophils of the peripheral blood was investigated in four patients with malignant non-Hodgkin's lymphoma after treatment with 24-h MTX infusions (500-790 mg/m2) followed by leukovorin rescue. The myeloid bone marrow cells were separated into two fractions, using a two-step discontinuous Percoll gradient with densities of 1.076 and 1.095 g/ml. The upper fraction consisted predominantly of immature bone marrow cells plus lymphocytes, and the lower fraction contained the mature myeloid bone marrow cells. Cells in the proliferating (immature) myeloid compartment took up and retained MTX to a much greater extent than the mature myeloid cells and the neutrophils. Two days after the MTX infusion no MTX was detected in the neutrophils in spite of a general rise in the total neutrophil count. MTX reappeared in the neutrophils on day 8 in concentrations not related to the concentrations in the proliferating myeloid cells during the MTX infusion. The time of reappearance of MTX in the neutrophils was in accordance with the time it takes for cells in the proliferating pool of the bone marrow to mature and be released into the circulation. No neutropenia was seen after the MTX infusions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0344-5704
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
42-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Methotrexate kinetics in myeloid bone marrow cells and peripheral neutrophils.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't