Linked Life Data

3812976

Source:http://linkedlifedata.com/resource/pubmed/id/3812976

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1987-3-13
pubmed:abstractText
Crystals of bovine brain calmodulin have been grown using a novel procedure which utilizes a mixture of alcohols as a precipitant. Crystals were grown by vapor diffusion at room temperature in the presence of 15% ethanol, 25% 2-methyl-2,4-pentanediol, 5.0 mM calcium chloride, and 10.0 mM sodium acetate buffer at pH 4.0. Crystals were visible within 4 h and grew to 0.75 X 0.3 X 0.125 mm in 2 days, without seeding. The crystals of calmodulin are isomorphous with those found by W. J. Cook, J. R. Dedman, A. R. Means, and C. E. Bugg (1980, J. Biol. Chem. 255, 8152-8153). The protein crystallizes in space group P1 with unit cell dimensions a = 29.92 A, b = 55.96 A, c = 24.75 A, alpha = 93.81 degrees, beta = 99.24 degrees, and gamma = 88.40 degrees.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
158
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
361-4
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Growing calmodulin crystals for X-ray diffraction studies at room temperature in 2 days.
pubmed:publicationType
Journal Article