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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1987-2-27
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pubmed:abstractText |
The mechanisms involved in the transport of tetraethylammonium (TEA) in the rabbit renal brush border were investigated by using membrane vesicles. Transport of [14C]TEA (0.2 mM) was measured by a rapid filtration method. We have reported previously that an imposed pH gradient (pHi = 6, pHo = 7.4) stimulates the uptake of TEA markedly, yielding a transient overshoot of 200% above the equilibrium value. Here we demonstrate that an overshoot of similar magnitude can also be obtained in the presence of indirectly induced proton gradients, via the naturally occurring Na+/H+ exchanger or via the artificial K+/H+ exchanger nigericin. The TEA exchange mechanism is shown to be electroneutral, temperature-dependent and saturable [Km, 0.328 (CL for P less than .05: 0.250-0.425) mM; Vmax, 2.13 (CL for P less than .05: 1.98-2.32) nmole/mg of protein X 15 sec]. Other organic cations interact with this exchange mechanism: mepiperphenidol and morphine both cis-inhibited and trans-stimulated TEA uptake. Quinine, which was the most potent inhibitor of TEA uptake (57% inhibition at 10(-5) M), did not trans-stimulate. The mechanism appears to be specific for organic cations inasmuch as the organic anion p-aminohippurate did not cis-inhibit or trans-stimulate TEA uptake. These results demonstrate the presence of a H+/TEA exchange mechanism in the rabbit renal brush border membrane showing many similarities with that shown to be present in the rat.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Morphine,
http://linkedlifedata.com/resource/pubmed/chemical/Nigericin,
http://linkedlifedata.com/resource/pubmed/chemical/Piperidines,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium-Hydrogen Antiporters,
http://linkedlifedata.com/resource/pubmed/chemical/Quinine,
http://linkedlifedata.com/resource/pubmed/chemical/Tetraethylammonium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/mepiperphenidol
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-3565
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
240
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
308-13
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3806393-Animals,
pubmed-meshheading:3806393-Carrier Proteins,
pubmed-meshheading:3806393-Dose-Response Relationship, Drug,
pubmed-meshheading:3806393-Female,
pubmed-meshheading:3806393-Hydrogen-Ion Concentration,
pubmed-meshheading:3806393-Kidney,
pubmed-meshheading:3806393-Kinetics,
pubmed-meshheading:3806393-Male,
pubmed-meshheading:3806393-Membrane Potentials,
pubmed-meshheading:3806393-Microvilli,
pubmed-meshheading:3806393-Morphine,
pubmed-meshheading:3806393-Nigericin,
pubmed-meshheading:3806393-Piperidines,
pubmed-meshheading:3806393-Potassium-Hydrogen Antiporters,
pubmed-meshheading:3806393-Quinine,
pubmed-meshheading:3806393-Rabbits,
pubmed-meshheading:3806393-Temperature,
pubmed-meshheading:3806393-Tetraethylammonium Compounds
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pubmed:year |
1987
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pubmed:articleTitle |
Tetraethylammonium transport in renal brush border membrane vesicles of the rabbit.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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