Linked Life Data

3803706

Source:http://linkedlifedata.com/resource/pubmed/id/3803706

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1987-3-6
pubmed:abstractText
The metabolism of thyroxine (T4) and triiodothyronine (T3) in cultured glial cells was studied in situ. Cultures were prepared from fetal rat brain and grown for the last 4 days in a chemically defined medium (CDM). They contained astrocytes and oligodendrocytes as shown by the enzyme markers, glutamine synthetase and 2',3'-cyclic nucleotide phosphohydrolase. These cells contained high affinity (22-33 pM), limited capacity (120-230 fmol/mg DNA) nuclear receptors for T3. Cells incubated in situ with 50 pM [125I]T4 actively metabolized the hormone. The major iodothyronine produced was T3 (220-570 fmol/4 h/mg DNA). About 70% accumulated in the cells, the remainder was released into the medium. Within the cells, T3 was partly bound to the nuclear receptors (16.5-20 fmol/mg DNA). Reverse T3 (rT3) was a minor metabolite (30-45 fmol/4 h/mg DNA); it was almost completely released into the medium. The half-life of [125I]T3 (50 pM) was found to be about 15 h. These results show that, in situ, glial cell cultures containing astrocytes and oligodendrocytes grown in CDM actively deiodinate T4 to T3 and degrade T3 rather slowly.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-78
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Thyroid hormone metabolism by glial cells in primary culture.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't