Linked Life Data

3803320

Source:http://linkedlifedata.com/resource/pubmed/id/3803320

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1987-3-26
pubmed:abstractText
We studied expression of the c-myc and c-ras protooncogenes during the initiation of pig thyroid cells in primary cell culture. This period is associated with major changes in differentiated thyroid cell function. After 1, 2, and 3 days of culture, polyadenylated mRNA was prepared from 30-50 replicate dishes of cells. mRNA was also prepared from a portion of the intact tissue used to prepare the dispersed follicles. Expression of c-myc and c-ras mRNA was determined by Northern blot analysis using v-myc and v-ras probes labeled by nick translation. As a nononcogene control, actin mRNA was determined using a beta-actin probe. In intact thyroid tissue before follicle dispersion, c-myc (2.6 kilobases) and c-ras (1.1 kilobases) mRNA were detectable, though at relatively low levels. c-myc mRNA expression increased in cultured cells, to 430%, 670%, and 330% of tissue levels on the first, second, and third days of culture, respectively. In contrast to c-myc, c-ras oncogene mRNA expression was not significantly altered during the 3-day culture period. beta-Action mRNA expression, like c-myc, increased to 560%, 810%, and 460% of tissue levels on the first, second, and third days of culture, respectively. Southern blot analysis of thyroid tissue and cultured cell DNA indicated that gene amplification did not account for the increase in c-myc mRNA levels in cultured cells. In conclusion, c-myc, but not c-ras, oncogene expression is enhanced during the transition of thyroid follicular cells into monolayer culture. beta-Actin mRNA expression is also enhanced during the initiation of primary thyroid cell culture. These data are consistent with a role for c-myc expression in the regulation of normal thyroid cell differentiated function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
120
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
924-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Initiation of normal thyroid cells in primary culture associated with enhanced c-myc messenger ribonucleic acid levels.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.