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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
23
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pubmed:dateCreated |
1987-3-16
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pubmed:abstractText |
The synthesis and kinetics characterization of a new class of dopamine beta-hydroxylase (DBH; EC 1.14.17.1) inhibitor, 1-(4-hydroxybenzyl)imidazole-2-thiol, is reported. These inhibitors, which incorporate a phenethylamine substrate mimic and an oxygen mimic into a single molecule, exhibit both the kinetic properties and the potency (Kis approximately 10(-9) M) expected for a multisubstrate inhibitor and are therefore classified as such. Steady-state kinetic experiments with these multisubstrate inhibitors and their substructural analogues support the recently proposed pH-dependent changes in substrate binding order [Ahn, N., & Klinman, J. P. (1983) Biochemistry 22, 3106] and a mechanism whereby the inhibitor binds specifically to the reduced Cu+ form of enzyme at both the phenethylamine substrate site and the active-site copper atom(s). A Yonetani-Theorell double-inhibition experiments indicates mutually exclusive binding of the inhibitor substructures p-cresol and 1-methylimidazole-2-thiol to suggest an extremely short intersite distance between the phenethylamine binding site and the active-site copper atom(s).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-2960
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7271-8
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:3801416-Animals,
pubmed-meshheading:3801416-Cattle,
pubmed-meshheading:3801416-Chromaffin Granules,
pubmed-meshheading:3801416-Dopamine beta-Hydroxylase,
pubmed-meshheading:3801416-Imidazoles,
pubmed-meshheading:3801416-Indicators and Reagents,
pubmed-meshheading:3801416-Kidney Medulla,
pubmed-meshheading:3801416-Kinetics,
pubmed-meshheading:3801416-Mathematics,
pubmed-meshheading:3801416-Structure-Activity Relationship
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pubmed:year |
1986
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pubmed:articleTitle |
Design and kinetic characterization of multisubstrate inhibitors of dopamine beta-hydroxylase.
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pubmed:publicationType |
Journal Article
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