Linked Life Data

3798526

Source:http://linkedlifedata.com/resource/pubmed/id/3798526

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1987-2-19
pubmed:abstractText
Four different murine and human thymic stromal cell (TSC) lines were analyzed by means of indirect immunofluorescence for the presence of tissue specific intermediate filament proteins, extracellular matrix products and thymic hormone. Among these cell lines, only one (IT-45R1) was shown to be epithelial in nature, as revealed by its immunoreactivity with antikeratin antibodies. In addition, IT-45R1 cells (but not the other cell lines) produced thymulin--a well defined thymic hormone--that was detected intracellularly and in the culture medium. Concerning extracellular matrix elements, we noted that all TSC lines were able to produce basement membrane proteins as type IV collagen and fibronectin. Type I collagen however was synthesized by TEPI, HT-5 and HT-7 cells but not by IT-45R1 cells. Our data demonstrate that only the IT-45R1 TSC line is epithelial and active in thymic hormone production. The other three TSC lines are not epithelial in nature, but their definitive phenotypes remain to be determined.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0165-6090
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-56
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Immunohistochemical studies on the phenotype of murine and human thymic stromal cell lines.
pubmed:publicationType
Journal Article