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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1979-9-25
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pubmed:abstractText |
A murine model was developed to study the cell-mediated immune response of mice immunized with one of two live, avirulent forms of Cryptococcus neoformans: a nonencapsulated mutant and a thinly encapsulated pseudohyphal variant. A lymphocyte transformation assay was used to evaluate the cellular response of control and sensitized spleen cells after in vitro incubation with three merthiolate-killed whole-cell antigens of C. neoformans. An antigen-to-spleen cell ratio of 10:1 and 5 days of incubation of antigen-spleen cell mixtures were established as optimal conditions for maximum lymphocyte transformation. Maximum responses occurred from 2 to 3 weeks after the last of eight weekly intraperitoneal inoculations of C. neoformans. This assay provided an accurate, reproducible method of studying cell-mediated immunity to C. neoformans, and applications to the study of cryptococcal pathogenesis are proposed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-13567788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-13763700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-339078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-346496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-352931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-4164846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-4569918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-4575001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-4619352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-5557280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-6057803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-631880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-76314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-780278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-785628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-786891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/378854-927451
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
449-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:378854-Animals,
pubmed-meshheading:378854-Cryptococcus,
pubmed-meshheading:378854-Cryptococcus neoformans,
pubmed-meshheading:378854-Dose-Response Relationship, Immunologic,
pubmed-meshheading:378854-Immunization,
pubmed-meshheading:378854-Kinetics,
pubmed-meshheading:378854-Lymphocyte Activation,
pubmed-meshheading:378854-Male,
pubmed-meshheading:378854-Mice,
pubmed-meshheading:378854-Spleen
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pubmed:year |
1979
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pubmed:articleTitle |
Kinetics of lymphocyte transformation in mice immunized with viable avirulent forms of Cryptococcus neoformans.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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