Linked Life Data

3786108

Source:http://linkedlifedata.com/resource/pubmed/id/3786108

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-1-20
pubmed:abstractText
Liposomes were prepared from mixed micelles by a dilution method. Mixed micellar solutions, containing constant octyl glucoside and egg phosphatidylcholine concentrations and varying amounts of cholesterol and/or a charged compound, were diluted at defined rates. After dilution, the resulting liposome dispersions were sequentially concentrated, washed or dialysed, and filtered. The effect of lipid composition and experimental conditions on physicochemical characteristics was studied. Fairly homogeneous liposome dispersions with mean diameters ranging from 100 to over 200 nm could be obtained. The particle size was dependent on cholesterol content and surface charge, and could be reproducibly controlled by adjustment of the dilution rate. Liposomes with a mean diameter below 100 nm could also be obtained, but were heterodisperse and unstable. The incorporation of charged compounds was monitored by microelectrophoresis. 31P-NMR measurements indicated that the liposomes were unilamellar. Dialysis appeared to be more convenient than washing to remove octyl glucoside.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0167-6555
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
259-65
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Preparation of liposomes via detergent removal from mixed micelles by dilution. The effect of bilayer composition and process parameters on liposome characteristics.
pubmed:publicationType
Journal Article