| pubmed-article:3785167 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3785167 | lifeskim:mentions | umls-concept:C0005595 | lld:lifeskim |
| pubmed-article:3785167 | lifeskim:mentions | umls-concept:C1708726 | lld:lifeskim |
| pubmed-article:3785167 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:3785167 | lifeskim:mentions | umls-concept:C0596963 | lld:lifeskim |
| pubmed-article:3785167 | lifeskim:mentions | umls-concept:C0002270 | lld:lifeskim |
| pubmed-article:3785167 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:3785167 | pubmed:dateCreated | 1987-1-14 | lld:pubmed |
| pubmed-article:3785167 | pubmed:abstractText | Toward understanding the controls affecting eucaryotic chromosome replication, we used a runoff replication assay to investigate whether the activity of a gene is related to its use of an upstream or downstream replication origin. When in vivo-initiated DNA polymerases are allowed to complete replication in vitro in the presence of bromodeoxyuridine triphosphate the density label is preferentially incorporated into origin-distal regions of DNA. Isopycnic centrifugation and blot hybridization analysis of the relative bromodeoxyuridine triphosphate incorporation into fragments spanning the chicken alpha-globin locus indicate that this region is replicated from an upstream origin both in chicken lymphocytes and in erythrocytes. Thus the replication polarity of these genes does not change as a function of transcriptional activity, consistent with earlier suggestions that DNA replication in the transcriptional direction may be a necessary but not sufficient condition for gene expression. | lld:pubmed |
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| pubmed-article:3785167 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:3785167 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3785167 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3785167 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:3785167 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:3785167 | pubmed:author | pubmed-author:JamesC DCD | lld:pubmed |
| pubmed-article:3785167 | pubmed:author | pubmed-author:LeffakMM | lld:pubmed |
| pubmed-article:3785167 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3785167 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:3785167 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3785167 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3785167 | pubmed:pagination | 976-84 | lld:pubmed |
| pubmed-article:3785167 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3785167 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3785167 | pubmed:articleTitle | Polarity of DNA replication through the avian alpha-globin locus. | lld:pubmed |
| pubmed-article:3785167 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3785167 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:3785167 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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