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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1987-1-14
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pubmed:abstractText |
Toward understanding the controls affecting eucaryotic chromosome replication, we used a runoff replication assay to investigate whether the activity of a gene is related to its use of an upstream or downstream replication origin. When in vivo-initiated DNA polymerases are allowed to complete replication in vitro in the presence of bromodeoxyuridine triphosphate the density label is preferentially incorporated into origin-distal regions of DNA. Isopycnic centrifugation and blot hybridization analysis of the relative bromodeoxyuridine triphosphate incorporation into fragments spanning the chicken alpha-globin locus indicate that this region is replicated from an upstream origin both in chicken lymphocytes and in erythrocytes. Thus the replication polarity of these genes does not change as a function of transcriptional activity, consistent with earlier suggestions that DNA replication in the transcriptional direction may be a necessary but not sufficient condition for gene expression.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3785167-1059262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3785167-1145202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3785167-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3785167-141375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3785167-2578323,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3785167-562720,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3785167-987858
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0270-7306
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
976-84
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
|
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pubmed:year |
1986
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pubmed:articleTitle |
Polarity of DNA replication through the avian alpha-globin locus.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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