Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1987-1-6
|
| pubmed:abstractText |
The DNA content of 72 bladder tumors and 20 healthy bladders was studied with flow cytometry. Reproducibility of DNA measurement was systematically verified with the use of 3 controls: calf thymocytes, mouse hepatocytes and a mixture of human lymphocytes with trout red blood cells. For each tumor or normal bladder, a first sample containing urothelial cells only and a second sample with the addition of trout red blood cells were systematically examined. In the first group of patients with healthy bladders the percentage of urothelial cells with a DNA content greater than G0 G1 (2N) was on average 7.9% +/- 2.8%. Among the second group of 72 tumors, 2 DNA profiles were remarkable: in a first group (39/72; 55%) DNA profile was unimodal (single diploid peak); in a second group (33/72; 45%) DNA profile was bimodal (association of a diploid peak with an aneuploid one). Comparison of flow data with pathology data (WHO classification) clearly demonstrated that the presence of an aneuploid peak, characterizes grade elevation and infiltration. Hence, 1/16 (6%) of grade I; 7/75 (28%) of grade II; 25/31 (81%) of grade III tumors show an aneuploid peak, as well as 11/44 (25%) of Ta; 9/14 (64%) of T1; 5/6 (83%) of T2, and 8/8 (100%) of T3 tumors. Demonstration of an aneuploid peak with flow cytometry particularly in the group of patients having non infiltrating Ta tumors (11/44 in our series) could further either lead to a closer follow-up of this high risk group of patients or to the early recommendation of a non conservative treatment.
|
| pubmed:language |
fre
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0248-0018
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
92
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
223-30
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1986
|
| pubmed:articleTitle |
[Study of the DNA content of 72 cases of bladder tumors using flow cytometry. Relation with anatomoclinical data].
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
English Abstract,
Research Support, Non-U.S. Gov't
|