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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1987-1-21
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pubmed:abstractText |
Isomeric dipeptides which contain proline at the C-terminus and undergo on-column cis-trans isomerization were separated by reversed-phase chromatography at subambient column temperature. Chromatography at low temperatures offers a convenient method for measuring the relative concentrations of the isomers and, thus, the calculation of the equilibrium constants for such cis-trans isomerization at various conditions of solvent, pH, and temperature. Pure fractions of the cis and trans isomers were collected and used in kinetic studies of the isomerization. High-performance liquid chromatography at temperatures near the freezing point of the eluent is a potentially useful tool for the study of a wide range of biochemical molecules.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9673
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
368
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
203-13
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3782366-Chromatography, High Pressure Liquid,
pubmed-meshheading:3782366-Dipeptides,
pubmed-meshheading:3782366-Hydrogen-Ion Concentration,
pubmed-meshheading:3782366-Isomerism,
pubmed-meshheading:3782366-Kinetics,
pubmed-meshheading:3782366-Proline,
pubmed-meshheading:3782366-Temperature
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pubmed:year |
1986
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pubmed:articleTitle |
Low temperature high-performance liquid chromatography of cis-trans proline dipeptides.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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