Linked Life Data

3777639

Source:http://linkedlifedata.com/resource/pubmed/id/3777639

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1986-12-9
pubmed:abstractText
Red blood cells were collected from Landrace X Duroc pigs in pooled and single batches. The RBC were stored for 24 hours to 20 days and exposed to 1 or more chemical and physical stressors. The chemicals were pyruvate, lactate, inosine, concanavalin A-luminol-bovine serum albumin conjugate, hydrogen peroxide, L-mimosine, and 3-amino-L-tyrosine. Physical stressors included thermogenic microwave radiation (2,450 MHz, mean specific absorption rate of 91 W/kg) and conventional heating with hot air or hot-water bath. Heating erythrocytes to 43 C for 10 minutes with microwaves or hot air did not significantly (P greater than 0.05) increase hemolysis, compared with hemolysis of RBC at 4 C (controls). Pyruvate or lactate did not affect the RBC under these conditions. Hemolysis of cells coated with concanavalin A-luminol-bovine serum albumin and heated to 43 C for 10 minutes by use of microwaves or hot air was significantly (P less than 0.05 by Student's t test) greater (48%) than that of RBC at 4 C (controls). The method of heating or the presence of pyruvate, lactate, or inosine did not have a significant (P greater than 0.05) effect on hemolysis. Lysis of RBC (14 days after collection) coated with concanavalin A-luminol-bovine serum albumin and stored at 4 C was not significantly different (P greater than 0.05) than that of noncoated cells (6 days after collection) stored at 4 C (control). When RBC were heated 20 days after collection to 48 C for 30 minutes, using a hot water bath, hemolysis was 60.6% greater than that of control cells (4 C).(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0002-9645
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2155-60
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Physiologic aging of mature porcine erythrocytes: effects of various metabolites, antimetabolites, and physical stressors.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.