Linked Life Data

3771572

Source:http://linkedlifedata.com/resource/pubmed/id/3771572

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
32
pubmed:dateCreated
1986-12-15
pubmed:abstractText
The cell surface cAMP receptor was excised from preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and used to generate a polyclonal antiserum. The antiserum immunoprecipitates the two molecular weight forms of the cAMP receptor. Both forms are phosphorylated. Western blot analyses show that the antiserum is highly specific and recognizes only the two molecular weight forms of the cAMP receptor. Immunological studies indicate that both forms of the receptor are phosphorylated. Vegetative amoebae possess low levels of the cAMP receptor. Levels of the antigen increase in differentiated cells which express high cell surface cAMP binding activity. The antiserum was also used to isolate 6 lambda gt11 cDNA clones. One of those clones contains a 1.1-kilobase pair cDNA fragment which encodes for a protein of approximately 30,000-35,000 daltons. The antibody which binds to the fusion protein also recognizes the two molecular weight forms of the receptor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
261
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15192-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Immunological analyses of the chemotactic receptor of Dictyosteleum discoideum. Identification of cDNA clones.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.