Linked Life Data

3768961

Source:http://linkedlifedata.com/resource/pubmed/id/3768961

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-12-11
pubmed:abstractText
Covalently closed circular (CCC) double-stranded DNA believed to be the transcriptional template for duck hepatitis B virus (DHBV) is amplified in aging primary cultures of hepatocytes from congenitally infected ducklings. Analysis of 5-bromodeoxyuridine-labeled heavy/light CCC DNA shows that the relaxed circular DNA synthesized in the cytoplasm by reverse transcription is the predominant precursor to the amplified pool of nuclear viral CCC DNA. In vitro infection of uninfected hepatocyte cultures with DHBV demonstrates that a similar 50-fold amplification of CCC DNA occurs during an early stage in the infection before virus production. This amplification allows the establishment of a pool of transcriptional templates in the cell without the need for semiconservative replication or multiple rounds of infection. This process may account for the ability of hepadnavirus-infected cells persistently to produce virus particles in the absence of stable integration of viral DNA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
451-60
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Formation of the pool of covalently closed circular viral DNA in hepadnavirus-infected cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.