Linked Life Data

3767957

Source:http://linkedlifedata.com/resource/pubmed/id/3767957

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-10-30
pubmed:abstractText
Prolonged sonication (3 h) of equimolar amounts of lysophosphatidylcholine (lysoPC) and cholesterol (chol) produces small unilamellar vesicles. Phosphorus-31 NMR (32.20 MHz) of the vesicles gave rise to a single peak (40.5 ppm) which was split upon addition of lanthanide ions. An additional, more intense signal appeared downfield near 51.0 ppm due to 2.4 mM Pr3+, upfield near 34.3 ppm due to 5 mM Yb3+. The more intense signals responsive to paramagnetic ions were assigned to lysoPC located in the outer vesicle leaflet; the signal not shifted by the ions was assigned to inside lysoPC. Based on peak intensities, an outside-to-inside lysoPC ratio (Ro/i) of 6.5-6.6 was determined. Essentially the same Ro/i values (6.6-6.8) were obtained when Pr3+ was present only in the vesicle interior or when Pr3+ was on the inside and Pr3+ and Yb3+ were on the outside. Ion leakage did not occur. Our data demonstrate that lysoPC/chol (1:1) vesicles are drastically asymmetric and that lysoPC shows a distinct preference for the outer bilayer leaflet.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
139
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
25-30
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Bilayer asymmetry in lysophosphatidylcholine/cholesterol (1:1) vesicles. A phosphorus-31 NMR study.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't