Linked Life Data

3767267

Source:http://linkedlifedata.com/resource/pubmed/id/3767267

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1986-11-14
pubmed:abstractText
A semi-automated method for determination of glycosylation of plasma proteins using the thiobarbituric acid method is described. Plasma samples (100 microL) and a plasma pool used as a secondary standard were incubated in 0.3 M oxalic acid at 100 degrees C for exactly 2 h. The hydroxymethyl furfural released and the total protein were measured concurrently on a Technicon AAII Autoanalyzer. Addition of 10 or 20 mmol glucose to plasma samples caused a minimal increase in the measured glycosylated protein. Within-batch and between-batch coefficients of variation were 3.1% and 4.9% respectively. The mean glycosylated protein levels for 52 normals and 48 maturity-onset diabetics were (+/- 1SD) 0.96 +/- 0.13 and 1.75 +/- 0.32 nmol HMF/mg protein/2 h incubation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0004-5632
pubmed:author
pubmed:issnType
Print
pubmed:volume
23 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
198-203
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Glycosylated plasma protein measurement by a semi-automated method.
pubmed:publicationType
Journal Article