Linked Life Data

376714

Source:http://linkedlifedata.com/resource/pubmed/id/376714

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1979-9-1
pubmed:abstractText
A biochemical parameter of lymphocyte activation, lipid synthesis, has been measured in a purified specific antigen-binding cell population (ABC). ABC isolated form immune and nonimmune animals by sequential centrifugation on buoyant density and sedimentation velocity gradients have a 2- to 7-fold higher rate of 14-C choline incorporation into phospholipid than either unfractionated spleen cells or cells depleted of ABC. Aslo ABC from immune animals were shown to have a 4- to 7-fold higher rate of 14C-acetate incorporation into their neutral lipids than nonbinding controls. The elevated lipid synthesis seen in both nonimmune SRBC-ABC and TNP-SRBC ABC indicates that antigenic contact via the B cell immunoglobulin receptor results in signal transduction and activation of the specific receptor-bearing lymphocyte population. Binding of the same particle (SRBC) to B cells via their Fc receptors did not regularly result in activation of lipid synthesis. The magnitude of the increased lipid synthesis in ABC populations approached that seen in LPS-stimulated spleen cells. We propose that the measurement of early activation events in purified ABC may be a more appropriate criterion for antigen-induced signals that later events such as thymidine incorporation or antibody secretion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1278-84
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Lipid synthesis: an indicator of antigen-induced signal transduction in antigen-binding cells.
pubmed:publicationType
Journal Article