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rdf:type |
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lifeskim:mentions |
umls-concept:C0004651,
umls-concept:C0012854,
umls-concept:C0014834,
umls-concept:C0205245,
umls-concept:C0208356,
umls-concept:C0243111,
umls-concept:C0311400,
umls-concept:C0449432,
umls-concept:C1179435,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1704675,
umls-concept:C1705248,
umls-concept:C1947951
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pubmed:issue |
2
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pubmed:dateCreated |
1979-8-29
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pubmed:abstractText |
T4 phage completely defective in both gene 30 (DNA ligase) and the rII gene (function unknown) require at least normal levels of host-derived DNA ligase (E. coli lig gene) for growth. Viable E. coli mutant strains that harbor less than 5% of the wild-type level of bacterial ligase do not support growth of T4 doubly defective in genes 30 and rII (T4 30- rII- mutants). We describe here two classes of secondary phage mutations that permit the growth of T4 30- rII- phage on ligase-defective hosts. One class mapped in T4 gene su30 (Krylov 1972) and improved T4 30- rII- phage growth on all E. coli strains, but to varying degrees that depended on levels of residual host ligase. Another class mapped in T4 gene 32 (helix-destabilizing protein) and improved growth specifically on a host carrying the lig2 mutation, but not on a host carrying another lig- lesion (lig4). Two conclusions are drawn from the work: (1) the role of DNA ligase in essential DNA metabolic processes in T4-infected E. coli is catalytic rather than stoichiometric, and (2) the E. coli DNA ligase is capable of specific functional interactions with components of the T4 DNA replication and/or repair apparatus.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-1165600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4351461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4377758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4444040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4563042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4588574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4589857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4591048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4731014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4896157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4910266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4912244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4922293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4939386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-4995816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-5340340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-791948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/376399-885343
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-89
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
Functional interactions beween the DNA ligase of Escherichia coli and components of the DNA metabolic apparatus of T4 bacteriophage.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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