Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-11-19
pubmed:abstractText
We have studied the calorimetric and infrared spectroscopic properties of the amino acid proline which has been implicated in the stabilization of biomacromolecules during reduced water states. It has been suggested that the ability of this molecule to protect biomacromolecules during these stress states may be related to the formation of polymeric aggregates of proline monomers in solution. The structure of this aggregate is thought to be an alternates stack, forming a hydrophilic colloid-like polymer which is thought to interact with hydrophobic moieties of biomacromolecules, reducing the exposed hydrophobic area during reduced water conditions. Calorimetric data presented in this work show that in increasing concentration of proline in solution the enthalpy associated with the melting of bulk water is greatly reduced, indicating strong hydrogen bonding character of proline in aqueous solution. Proline shows two eutectic phase separations at moderate concentrations and one of these eutectics may be the proposed intermolecular state. A partial phase diagram for proline is presented. Fourier-transform infrared spectroscopic data indicate that the COO- asymmetric stretch of proline shows marked splitting with increasing proline concentration. This suggests that the carboxylate is in different environments, with the high energy vibrations representing COO- groups which are participating in the hydrogen bonding pattern associated with the formation of the intermolecular stack. Changes in the CH2 asymmetric and symmetric stretches of the pyrrolidine rings of proline are consistent with the proposed stack structure. We also suggest a possible mechanism by which these intermolecular associations may be important in the protection of biomacromolecules during reduced water states.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-16250339, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-16661033, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-16663049, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-16827211, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-3947095, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-4028782, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-5044066, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-5159797, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-5469157, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-6372974, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-6391546, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-667127, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-6742860, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-6884075, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-700152, http://linkedlifedata.com/resource/pubmed/commentcorrection/3756295-916702
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
423-30
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
A calorimetric and infrared spectroscopic study of the stabilizing solute proline.
pubmed:publicationType
Journal Article, Comparative Study