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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1979-7-25
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pubmed:abstractText |
Certain human antinuclear antibodies (ANA) cross-react specifically with the surface membrane of human leucocytes. In the present work these cross-reacting (X) ANA could be eluted directly from viable leucocytes isolated from three patients. The plasma membrane antigen recognized by X-ANA appeared phylogenetically highly conserved since it was present on viable HeLa cells and cells from mouse and rabbit spleen, rat liver, and the slime mould Physarum polycephalum. X-ANA did not react with the plasma membrane of nucleated bird erythrocytes or human erythrocytes or thrombocytes. The same X-ANA subset reacted with the various cells. The presence of X-ANA correlated strongly (P less than 0.01) with antibodies against a HeLa cell surface antigen. The nuclear antigen recognized by X-ANA was present in all species examined, including bird erythrocyte nuclei and nuclei of Physarum polycephalum. Native or denatured DNA, RNA or a saline extract of nuclei did not inhibit the binding of X-ANA to cell nuclei.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0300-9475
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
325-32
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:375379-Animals,
pubmed-meshheading:375379-Antibodies, Antinuclear,
pubmed-meshheading:375379-Antigens, Surface,
pubmed-meshheading:375379-Cell Membrane,
pubmed-meshheading:375379-Cell Nucleus,
pubmed-meshheading:375379-Cross Reactions,
pubmed-meshheading:375379-DNA,
pubmed-meshheading:375379-Fluorescent Antibody Technique,
pubmed-meshheading:375379-HeLa Cells,
pubmed-meshheading:375379-Humans,
pubmed-meshheading:375379-Leukocytes,
pubmed-meshheading:375379-Physarum,
pubmed-meshheading:375379-RNA,
pubmed-meshheading:375379-Rabbits,
pubmed-meshheading:375379-Rats,
pubmed-meshheading:375379-Receptors, Antigen, B-Cell,
pubmed-meshheading:375379-Species Specificity
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pubmed:year |
1979
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pubmed:articleTitle |
Properties of antinuclear antibodies that cross-react with plasma membranes.
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pubmed:publicationType |
Journal Article
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