Linked Life Data

375221

Source:http://linkedlifedata.com/resource/pubmed/id/375221

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1979-7-25
pubmed:abstractText
A set of vector DNAs (Y vectors) useful for the cloning of DNA fragments in Saccharomyces cerevisiae (yeast) and in Escherichia coli are characterized. With these vectors, three modes of yeast transformation are defined. (i) Vectors containing yeast chromosomal DNA sequences (YIp1, YIp5) transform yeast cells at low frequency (1--10 colonies per microgram) and integrate into the genome by homologous recombination; this recombination is reversible. (ii) Hybrids containing endogenous yeast plasmid DNA sequences (YEp2, YEp6) transform yeast cells at much higher frequency (5000--20,000 colonies per microgram). Such molecules replicate autonomously with an average copy number of 5--10 covalently closed circles per yeast cell and also replicate as a chromosomally integrated structure. This DNA may be physically isolated in intact form from either yeast or E. coli and used to transform either organism at high frequency. (iii) Vectors containing a 1.4-kilobase yeast DNA fragment that includes the centromere linked trp1 gene (YRp7) transform yeast with an efficiency of 500--5000 colonies per microgram; such molecules behave as minichromosomes because they replicate autonomously but do not integrate into the genome. The uses of Y vectors for the following genetic manipulations in yeast are discussed: isolation of genes; construction of haploid strains that are merodiploid for a particular DNA sequence; and directed alterations of the yeast genome. General methods for the selection and the analysis of these events are presented.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/375221-319344, http://linkedlifedata.com/resource/pubmed/commentcorrection/375221-322128, http://linkedlifedata.com/resource/pubmed/commentcorrection/375221-331256, http://linkedlifedata.com/resource/pubmed/commentcorrection/375221-339098, http://linkedlifedata.com/resource/pubmed/commentcorrection/375221-341150, http://linkedlifedata.com/resource/pubmed/commentcorrection/375221-344137, http://linkedlifedata.com/resource/pubmed/commentcorrection/375221-347451, http://linkedlifedata.com/resource/pubmed/commentcorrection/375221-4216403, http://linkedlifedata.com/resource/pubmed/commentcorrection/375221-4551142, http://linkedlifedata.com/resource/pubmed/commentcorrection/375221-4945859, http://linkedlifedata.com/resource/pubmed/commentcorrection/375221-775490
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1035-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.