Linked Life Data

3743889

Source:http://linkedlifedata.com/resource/pubmed/id/3743889

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1986-10-15
pubmed:abstractText
When dissociated anterior pituitary cells were cultured over a layer of gelatin, electron microscopic observation showed the mammotrophs in the culture to be essentially degranulated and to have massively dilated rough endoplasmic reticulum (RER) and Golgi apparatus (GA), while there were no discernible effects on any of the other cell types. PRL synthesis in the gelatin-cultured cells occurred at twice the rate of that in the control cells and the gelatin-cultured cells contained 3% of the normal amount of PRL. Transfer of the cells to a more traditional culture situation reversed most of the morphological changes within 24 h. The mammotrophs were responsive to thyrotropin-releasing hormone (10(-7) M) both immediately after gelatin culture (3-fold control) and after reversal (5-fold control). As placement of the gelatin-cultured cells into traditional culture synchronizes the onset of granulation, we conclude that mammotrophs cultured in this fashion will be useful in the further study of PRL synthesis, packaging, storage and degradation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-80
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Specific and reversible degranulation of pituitary mammotrophs.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.