Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1979-7-16
pubmed:abstractText
Escherichia coli K-12 minicells were employed to examine polypeptides encoded by plasmids carrying wild-type and mutant Tn1 or Tn3 transposition elements. Tn1- and Tn3-containing minicells express high levels of four transposon-specified polypeptides. Three, of molecular weights 30,000, 28,000, and 25,000, are related immunologically to beta-lactamase, the enzyme responsible for ampicillin hydrolysis. A fourth polypeptide of molecular weight 19,000 is encoded by the Tn1 or Tn3 region which spans the BamHI cleavage site. Mutant transposons which no longer produce this polypeptide transpose at higher than wild-type frequencies to give aberrant transposition products (Gill et al., J. Bacteriol. 136: 742--756, 1978; Heffron et al., Proc. Natl. Acad. Sci U.S.A. 72:3632--3627, 1975). No expression could be detected from a region of the transposons extending from the inverted repeat sequence distal to the beta-lactamase gene to more than half the distance into the Tn1 or Tn3 sequence.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-1059152, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-1061090, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-1093180, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-1102604, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-1102715, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-1103125, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-1103150, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-1267736, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-233723, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-233726, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-322140, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-327263, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-344137, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-358199, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-358200, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-361721, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-4559594, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-4609125, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-4760132, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-767328, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-773363, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-789346, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-792710, http://linkedlifedata.com/resource/pubmed/commentcorrection/374376-794689
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
48-54
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Polypeptides expressed in Escherichia coli K-12 minicells by transposition elements Tn1 and Tn3.
pubmed:publicationType
Journal Article