Linked Life Data

3731446

Source:http://linkedlifedata.com/resource/pubmed/id/3731446

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1986-9-17
pubmed:abstractText
We report a new fluorometric assay in which a fluorescent triglyceride is used for determining lipase activity in serum, and we compare it with turbidimetric and radiometric methods. Because this fluorometric method is at least 50-fold more sensitive than the turbidimetric method, we have been able to develop a micromethod that requires only very small amounts of substrate reagent and serum. The use of fluorescent-labeled fatty acids allows direct determination of the product of lipase action and obviates the use of a standard for calibrating the method. Results of the fluorometric method are well correlated with those of the radiometric and turbidimetric methods, and the precision of the fluorometric assay is better than that of the turbidimetric method. Reference values for normal subjects are between 0 and 120 mU/L.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0009-9147
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1532-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Fluorometric assay for pancreatic lipase.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't