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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
26
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pubmed:dateCreated |
1986-8-8
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pubmed:abstractText |
Uptake and release of kyotorphin (Tyr-Arg) in rat brain synaptosomes were studied. Synthetic kyotorphin was taken up into crude synaptosomes (P2), in a temperature-dependent manner. The Km and Vmax of the uptake were 1.31 +/- 0.12 X 10(-4) M and 5.9 +/- 0.5 pmol/mg protein/min, respectively. Metabolic inhibitors such as dinitrophenol and iodoacetamide and ouabain which is known as an inhibitor of Na+ dependent uptake mechanism significantly inhibited the uptake. When the synaptosomes previously preloaded with synthetic kyotorphin at 10(-4)M were exposed to high K+ medium, kyotorphin was released in a Ca2+-dependent manner. These findings support the view that kyotorphin plays a role as neurotransmitter/neuroregulator.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0024-3205
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2405-11
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3724363-Animals,
pubmed-meshheading:3724363-Biological Transport, Active,
pubmed-meshheading:3724363-Brain,
pubmed-meshheading:3724363-Calcium,
pubmed-meshheading:3724363-Depression, Chemical,
pubmed-meshheading:3724363-Dinitrophenols,
pubmed-meshheading:3724363-Endorphins,
pubmed-meshheading:3724363-Iodoacetamide,
pubmed-meshheading:3724363-Kinetics,
pubmed-meshheading:3724363-Ouabain,
pubmed-meshheading:3724363-Potassium,
pubmed-meshheading:3724363-Rats,
pubmed-meshheading:3724363-Rats, Inbred Strains,
pubmed-meshheading:3724363-Synaptosomes
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pubmed:year |
1986
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pubmed:articleTitle |
Uptake and release of kyotorphin in rat brain synaptosomes.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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