Linked Life Data

3723140

Source:http://linkedlifedata.com/resource/pubmed/id/3723140

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-7-25
pubmed:abstractText
A striking clinical response of hairy cell leukemia (HCL) to alpha-interferon (alpha-IFN) has recently been reported. To explain its efficacy on HCL, we investigated the ultrastructural changes caused by alpha-IFN in hairy cells. Mononuclear cells (MNCs) were separated from the peripheral blood of eight HCL patients in the leukemic phase. Aliquots of 1 X 10(6) MNCs in 2.5 ml of RPMI 1640 medium were cultured with a recombinant DNA-derived alpha-2 interferon (RD alpha 2-IFN) for 1, 3, and 7 days. The concentrations of IFN added were 10, 100, 1,000, and 10,000 IU/ml culture medium. Electron microscopic examination revealed that RD alpha 2-IFN induced the formation of tubuloreticular structures in cultured hairy cells and increased the frequency of hairy cells having annulate lamellae (ALs), probable precursors of the nuclear pore. Hairy cells from various patients showed a variability in the extent of tubuloreticular structure (TRS) formation. Excellent clinical responses, as judged by hematologic parameters, were observed among patients whose leukemic cells were susceptible to RD alpha 2-IFN with respect to TRS formation in vitro. from these findings, we propose that the induction of TRS formation, in combination with the increase of ALs, may be a morphologic expression of a direct antitumor action of IFN on malignant cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0732-6580
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
270-81
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Responses of hairy cell leukemia cells to a recombinant alpha-2 interferon in vitro and in vivo: correlation between formation of tubuloreticular structures and clinical parameters.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't