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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1986-8-20
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pubmed:abstractText |
The exposure of several major red-cell glycolipids to galactose oxidase was studied by oxidizing the cells with the enzyme and reducing them with NaB2H4. After isolation, the deuterium label was detected by mass fragmentography. 60-70% globoside in human and porcine erythrocytes was exposed as measured by this method. In contrast, asialo-GM2 in guinea-pig erythrocytes and Forssman glycolipid in sheep erythrocytes were mainly in a cryptic state. Neuraminidase treatment increased the incorporation of deuterium label to asialo-GM2 4-8-fold. A similar effect was seen in Forssman glycolipid when sheep red cells were labeled with the neuraminidase/galactose oxidase/NaB3H4 method. In contrast, the increase in labeling was only about 10-40% in porcine and human globosides, which were efficiently exposed to galactose oxidase already in native red cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Borohydrides,
http://linkedlifedata.com/resource/pubmed/chemical/Deuterium,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Forssman glycolipid,
http://linkedlifedata.com/resource/pubmed/chemical/Galactose Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Gangliosides,
http://linkedlifedata.com/resource/pubmed/chemical/Globosides,
http://linkedlifedata.com/resource/pubmed/chemical/Glycolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosphingolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neuraminidase,
http://linkedlifedata.com/resource/pubmed/chemical/ganglio-N-triaosylceramide,
http://linkedlifedata.com/resource/pubmed/chemical/sodium borohydride
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0014-2956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
157
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
611-6
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3720746-Animals,
pubmed-meshheading:3720746-Borohydrides,
pubmed-meshheading:3720746-Chromatography, Thin Layer,
pubmed-meshheading:3720746-Deuterium,
pubmed-meshheading:3720746-Erythrocytes,
pubmed-meshheading:3720746-Fatty Acids,
pubmed-meshheading:3720746-Galactose Oxidase,
pubmed-meshheading:3720746-Gangliosides,
pubmed-meshheading:3720746-Globosides,
pubmed-meshheading:3720746-Glycolipids,
pubmed-meshheading:3720746-Glycosphingolipids,
pubmed-meshheading:3720746-Guinea Pigs,
pubmed-meshheading:3720746-Humans,
pubmed-meshheading:3720746-Membrane Proteins,
pubmed-meshheading:3720746-Neuraminidase,
pubmed-meshheading:3720746-Oxidation-Reduction,
pubmed-meshheading:3720746-Sheep,
pubmed-meshheading:3720746-Swine
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pubmed:year |
1986
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pubmed:articleTitle |
Exposure of major neutral glycolipids in red cells to galactose oxidase. Effect of neuraminidase.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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