Linked Life Data

3710080

Source:http://linkedlifedata.com/resource/pubmed/id/3710080

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-7-7
pubmed:abstractText
Basolateral, brush-border, and Golgi-enriched subcellular membrane fractions, prepared from homogenates of rat small intestinal mucosa obtained by scraping, had unusually high concentrations of nonesterified fatty acids. These fatty acids appear to be responsible for the large amount of calcium binding, an effect that previously was shown to be reduced in vitamin D deficiency. In contrast, basolateral and Golgi membranes prepared from isolated cells had low levels of nonesterified fatty acids and calcium binding. Intermediate levels were found with isolated cells that were not put through the usual washing procedures. Addition to homogenates of scrapings of a lipase inhibitor, diethyl-p-nitrophenyl phosphate, reduced calcium binding and nonesterified fatty acids to levels similar to those in membranes prepared from isolated cells. Phospholipase A activity was low in homogenates of isolated cells and high in scrapings; this was reduced in intestinal scrapings of vitamin D-deficient rats. Ileal membranes had more calcium binding than duodenal membranes, and ileal homogenates also had greater phospholipase A activity. Preparation of subcellular membranes from rat intestinal scrapings can result in altered lipid composition, probably due to lipolytic enzyme activity; in addition to increasing cation-binding, these high levels of fatty acids may affect other membrane properties and enzyme function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0016-5085
pubmed:author
pubmed:issnType
Print
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
34-40
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Preparation of subcellular membranes from rat intestinal scrapings or isolated cells. Different Ca2+ binding, nonesterified fatty acid levels, and lipolytic activity.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't