Linked Life Data

3706737

Source:http://linkedlifedata.com/resource/pubmed/id/3706737

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-6-16
pubmed:abstractText
Protein hydrophobicity is often detected by binding of protein to micelles of a mild detergent. A new fluorescence method for detection of this binding is presented. The method is based on a long-range quenching of tryptophan fluorescence by energy transfer to a pyrene-labeled phospholipid probe incorporated into micelles of Brij 96. The method is rapid, simple, and requires only a few micrograms of protein. Strongest quenching is obtained when both pyrene probe and brominated Brij 96, a short-range quencher, are combined. To define the best assay conditions the physical properties and quenching behavior of micelles with or without these probes have been compared. It is shown that both quenchers accurately measure binding of model compounds and protein toxins to micelles. Comparison of quenching by the different probes can be used to derive information on tryptophan location relative to the micelle core.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:volume
154
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-63
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
A fluorescence-based detergent binding assay for protein hydrophobicity.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't