Linked Life Data

3705083

Source:http://linkedlifedata.com/resource/pubmed/id/3705083

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1986-5-30
pubmed:abstractText
The administration of 2,3,7,8-TCDD to 2-week-old White Leghorn cockerels produced a dose-dependent induction of hepatic microsomal ethoxyresorufin O-deethylase (EROD) and benzo[alpha]pyrene (B[alpha]P) hydroxylase activities with induction EC50 values of 778 and 302 ng/kg, respectively. In addition, 2,3,7,8-TCDD also induced 4-dimethylaminoantipyrine (DMAP) N-demethylase (EC50 = 561 ng/kg), this result contrasting with studies reported for other animal species in which 2,3,7,8-TCDD either does not induce or inhibits this cytochrome P-450 dependent monooxygenase enzyme activity. However, the reduced cytochrome P-450:CO binding difference spectral absorption maxima for the 2,3,7,8-TCDD induced microsomes was observed at 448 nm which was similar to that reported for most animals which have been investigated. Electrophoresis of control and 2,3,7,8-TCDD induced microsomal proteins using SDS polyacrylamide slab gels showed intensification of 3 protein staining bands at Mr 53 000, 56 000 and 58 000. Incubation of the 2,3,7,8-TCDD-induced microsomes with SKF-525A and alpha-naphthoflavone showed that both compounds inhibited DMAP N-demethylase, EROD and AHH and that alpha-naphthoflavone was the more potent inhibitor of all 3 microsomal monooxygenases. 2,3,7,8-TCDD treatment caused significant involution of the Bursa of Fabricius when administered at a dose level of 10 micrograms/kg for 3 days and this result confirmed the extreme sensitivity of the immature White Leghorn cockerel to the biologic and toxic effects elicited by 2,3,7,8-TCDD. However, in contrast to other sensitive species, no high affinity cytosolic receptor protein for [3H]2,3,7,8-TCDD could be detected in the liver of chick embryos or 2-week-old birds.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0300-483X
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
197-206
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:3705083-Animals, pubmed-meshheading:3705083-Aryl Hydrocarbon Hydroxylases, pubmed-meshheading:3705083-Benzopyrene Hydroxylase, pubmed-meshheading:3705083-Centrifugation, Density Gradient, pubmed-meshheading:3705083-Chickens, pubmed-meshheading:3705083-Cytochrome P-450 CYP1A1, pubmed-meshheading:3705083-Cytosol, pubmed-meshheading:3705083-Dioxins, pubmed-meshheading:3705083-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:3705083-Enzyme Induction, pubmed-meshheading:3705083-Injections, Intraperitoneal, pubmed-meshheading:3705083-Male, pubmed-meshheading:3705083-Microsomes, Liver, pubmed-meshheading:3705083-Mixed Function Oxygenases, pubmed-meshheading:3705083-Oxidoreductases, pubmed-meshheading:3705083-Oxidoreductases, N-Demethylating, pubmed-meshheading:3705083-Tetrachlorodibenzodioxin
pubmed:year
1986
pubmed:articleTitle
The biologic and toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin in chickens.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't